Methods for attaching proteins to lipidic microparticles with high efficiency
AssigneeThe Regents of the University of California
InventorsDemetrios Papahadjopoulos, Keelung Hong, Weiwen Zheng, Dmitri B. Kirpotin
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Abstract
The invention provides methods of attaching proteins to lipidic microparticles with efficiencies of at least 77%. The proteins are attached to linker molecules comprising a hydrophilic domain and a hydrophobic domain. The proteins can be antibodies, antibody fragments, hormones, growth factors, enzymes, or nucleic acid binding proteins, or other proteins. The proteins can be chemically conjugated to the linker molecules, or they can be fused to the linker molecules by recombinant techniques.
Claims16
A method for attaching proteins to lipidic microparticles with an efficiency of at least 77%, said method comprising
- (a) providing proteins, each conjugated to a terminus of a hydrophilic polymer chain, which hydrophilic polymer chain is attached to a hydrophobic domain terminally attached to the hydrophilic domain at a terminus contralateral to said protein, and
- (b) incubating a plurality of said conjugated proteins with said lipidic microparticles for a time sufficient to permit the hydrophobic domains attached to said hydrophilic domains conjugated to said proteins to become stably associated with the lipidic microparticles, thereby attaching the proteins to said lipidic microparticles.
A method for attaching proteins to lipidic microparticles with an efficiency of at least 77%, said method comprising
- (a) providing proteins, each comprising a terminally appended amino acid sequence comprising primarily amino acids with hydrophilic side chains, which sequence is followed by a synthetically appended lipid moiety, and
- (b) incubating said proteins with said lipidic microparticles for a time sufficient to permit the lipid moieties to become stably associated with the lipidic microparticles, thereby attaching the proteins to said lipidic microparticles.
Dependencies are inferred from the claim text.