Targeting molecule linked to an imaging agent
AssigneeBiolex Newco I, Inc.
InventorsAndrew C. Hiatt, Mich B. Hein, John H. Fitchen
Life of this patent
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Abstract
Targeting molecules are provided for use in delivering imaging agents to epithelial tissue. The targeting molecule comprises a polypeptide that forms a closed covalent loop, contains at least three peptide domains having β-sheet character, each of the domains being separated by domains lacking β-sheet character. The targeting molecule specifically binds to a basolateral factor attached to a basolateral domain of an epithelial cell surface causing internalization of a linked imaging agent into the cells. The polypeptide or imaging agent may be linked to a peptide amino acid sequence that directs delivery of the imaging agent to a carcinoma cell, a nucleus, or an endoplasmic reticulum.
Claims11
A targeting molecule capable of specifically binding to a basolateral factor attached to a basolateral domain of an epithelial cell surface and causing the internalization of an imaging agent linked thereto, wherein said targeting molecule consists of Domain 2 of a J chain.
A targeting molecule linked to at least one imaging agent, wherein said targeting molecule comprises a polypeptide that:
- (a) forms a closed covalent loop;
- and (b) contains at least three peptide domains having beta-sheet character, each of the domains being separated by domains lacking beta-sheet character;
- wherein said targeting molecule is linked to at least one imaging agent by a substrate for an intracellular or extracellular enzyme in or on an epithelial cell or epithelial cell surface, and wherein the targeting molecule does not contain an IgA heavy chain containing CH1α, CH2α, and CH3α domains and an IgA light chain containing a CL domain.
A targeting molecule linked to at least one imaging agent, wherein said targeting molecule comprises a polypeptide that:
- (a) forms a closed covalent loop;
- and (b) contains at least three peptide domains having beta-sheet character, each of the domains being separated by domains lacking beta-sheet character;
- wherein said targeting molecule is linked to at least one imaging agent through a side chain of amino acids in an antibody combining site, and wherein the targeting molecule does not contain an IgA heavy chain containing CH1α, CH2α, and CH3α domains and an IgA light chain containing a CL domain.
A targeting molecule linked to at least one imaging agent, wherein said targeting molecule comprises a polypeptide that:
- (a) forms a closed covalent loop;
- and (b) contains at least three peptide domains having beta-sheet character, each of the domains being separated by domains lacking beta-sheet character;
- wherein the targeting molecule does not contain an IgA heavy chain containing CH1α, CH2α, and CH3α domains and an IgA light chain containing a CL domain;
- wherein the imaging agent is not naturally linked with the targeting molecule, and wherein the imaging agent is not iodine.
A targeting molecule linked to at least one imaging agent, wherein said targeting molecule comprises a polypeptide that:
- (a) forms a closed covalent loop;
- and (b) contains at least three peptide domains having beta-sheet character, each of the domains being separated by domains lacking beta-sheet character, wherein the targeting molecule does not contain an IgA heavy chain containing CH1α, CH2α, and CH3α domains and an IgA light chain containing a CL domain.
Dependencies are inferred from the claim text.