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US 7,018,846 B1Granted
Display of receptors and analysis of binding interactions and drug libraries
AssigneeScience & Technology Corporation @ UNM
InventorsLarry A. Sklar, Eric Prossnitz, Janeen Vilven, Donna Neldon
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Abstract
A display and method of preparing 7-transmembrane and other receptors for real-time kinetic analysis of binding interactions. The invention includes display on beads and in micelles for multi-well and flow cytometric analysis. The invention is useful for ligand discovery and drug action discovery, and G-protein response in particular.
Claims24
A method for non-cellular analysis and display of 7-transmembrane receptors comprising the following steps:
- a) incorporating an attachment tether to a receptor;
- b) solubilizing the receptor;
- and
- c) presenting the receptor in conjunction with a support, wherein said support comprises at least one substrate selected from the group consisting of silica bead substrates, latex bead substrates and other bead substrates appropriate for flow cytometry, and wherein the receptor in conjunction with a support is analyzed with a flow cytometer in real-time.
1 more element
A method for non-cellular display of 7-transmembrane receptors comprising the following steps:
- a) incorporating an attachment means to a receptor;
- b) solubilizing the receptor;
- c) presenting the receptor in conjunction with a support;
- d) presenting at least one ligand to bind to the receptor, wherein said ligand is known to bind to the receptor;
- e) combining the receptor and ligand to accomplish binding while the receptor is bound to the support;
- and
- f) sorting the bound receptor ligand pairs by fluorescence and using flow cytometry to analyze the fluorescence and binding interactions in real-time.
4 more elements
Dependencies are inferred from the claim text.