Compositions and methods for the inhibition of neurotransmitter uptake of synaptic vesicles
AssigneeThe Regents of the University of Michigan
InventorsTetsufumi Ueda, Eric D. Özkan
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Abstract
Compositions and methods for treating neurosynaptic disorder in a subject are described. More specifically, compositions and methods for inhibiting glutamate uptake by synaptic vesicles in a subject are set forth. In one embodiment, the composition is inhibitory protein factor (IPF) and the subject is a human.
Claims14
A method for assessing overcoming synaptic vesicle glutamate uptake inhibition activity, comprising
- a) providing:
- i) synaptic vesicles,
- ii) a composition comprising a purified fordin fragment having glutamate uptake inhibition activity, said fragment having an N-terminus and a C-terminus, wherein said N-terminus is Tyr26 of fodrin, and
- iii) a candidate compound;
- and
- b) combining said candidate compound with said synaptic vesicles and said fragment such that the effect of said candidate compound on glutamate uptake by said vesicle can be assessed.
A method for assessing overcoming synaptic vesicle glutamate uptake inhibition activity, comprising
- a) providing:
- i) synaptic vesicles,
- ii) a composition comprising a purified fragment of IPF having synaptic vesicle glutamate uptake inhibition activity, said fragment having an N-terminus and a C-terminus, wherein said N-terminus is Tyr26 of fodrin, and
- iii) a candidate compound;
- and
- b) combining said candidate compound with said synaptic vesicles and said fragment such that the effect of said candidate compound on glutamate uptake by said vesicle can be assessed.
A method for assessing overcoming synaptic vesicle glutamate uptake inhibition activity, comprising:
- a) providing:
- i) synaptic vesicles,
- ii) a composition comprising a purified fragment of fodrin having glutamate uptake inhibition activity, said fragment having an N-terminus and a C-terminus, wherein said purified fragment comprises a peptide having the amino acid sequence EAALTSEEVG within 150 amino acids of the C-terminus of the peptide, and
- iii) a candidate compound;
- and
- b) combining said candidate compound with said synaptic vesicles and said fragment such that the effect of said candidate compound on glutamate uptake by said synaptic vesicles can be assessed.
A method for assessing overcoming synaptic vesicle glutamate uptake inhibition activity, comprising:
- a) providing:
- i) synaptic vesicles,
- ii) a composition comprising a purified peptide having glutamate uptake inhibition activity with an N-terminus sequence comprising the amino acid sequence YHRFK, and
- iii) a candidate compound;
- and
- b) combining said candidate compound with said synaptic vesicles and said fragment such that the effect of said candidate compound on glutamate uptake by said synaptic vesicles can be assessed.
Dependencies are inferred from the claim text.