P-TEFb compositions, methods and screening assays

An isolated nucleic acid consisting of a nucleic acid that encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:4, a nucleic acid that encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:45, a nucleic acid that encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:47, or a nucleic acid that encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO:50.

An isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:4, SEQ ID NO:45, SEQ ID NO:47 or SEQ ID NO:50.

An isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a P-TEFb large subunit protein, wherein said P-TEFb large subunit protein binds to a P-TEFb kinase subunit protein having the sequence of SEQ ID NO:2 or SEQ ID NO:6 to form a P-TEFb enzyme complex that promotes transcription elongation and wherein said nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:44.

An isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a P-TEFb large subunit protein, wherein said P-TEFb large subunit protein binds to a P-TEFb kinase subunit protein having the sequence of SEQ ID NO:2 or SEQ ID NO:6 to form a P-TEFb enzyme complex that promotes transcription elongation and wherein said nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:46.

An isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a P-TEFb large subunit protein, wherein said P-TEFb large subunit protein binds to a P-TEFb kinase subunit protein having the sequence of SEQ ID NO:2 or SEQ ID NO:6 to form a P-TEFb enzyme complex that promotes transcription elongation and wherein said nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:49.

An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:44, SEQ ID NO:46 or SEQ ID NO:49.

An isolated nucleic acid molecule comprising:

• (a) a first nucleic acid sequence that encodes a P-TEFb small subunit protein that has kinase activity and binds to a P-TEFb large subunit protein of SEQ ID NO:4, SEQ ID NO:45, SEQ ID NO:47 or SEQ ID NO:50 to form a P-TEFb enzyme complex that promotes transcription elongation, wherein said P-TEFb small subunit protein has the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:6;
• and
• (b) a second nucleic acid sequence that encodes a P-TEFb large subunit protein that binds to a P-TEFb kinase subunit protein of SEQ ID NO:2 or SEQ ID NO:6 to form a P-TEFb enzyme complex that promotes transcription elongation, wherein said P-TEFb large subunit protein has the amino acid sequence set forth in SEQ ID NO:4, SEQ ID NO:45, SEQ ID NO:47 or SEQ ID NO:50.

One or more expression units comprising:

• (a) a first isolated expression unit comprising, under the transcriptional control of a promoter, a first nucleic acid sequence that encodes a P-TEFb small subunit protein that has kinase activity and binds to a P-TEFb large subunit protein of SEQ ID NO:4, SEQ ID NO:45, SEQ ID NO:47 or SEQ ID NO:50 to form a P-TEFb enzyme complex that promotes transcription elongation, wherein said P-TEFb small subunit protein has the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:6;
• and
• (b) a second isolated expression unit comprising, under the transcriptional control of a promoter, a second nucleic acid sequence that encodes a P-TEFb large subunit protein that binds to a P-TEFb kinase subunit protein of SEQ ID NO:2 or SEQ ID NO:6 to form a P-TEFb enzyme complex that promotes transcription elongation, wherein said P-TEFb large subunit protein has the amino acid sequence of SEQ ID NO:4, SEQ ID NO:45, SEQ ID NO:47 or SEQ ID NO:50.

An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:44.

An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:46.

An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:49.