Recombinant double-stranded RNA phage, and use of the same
OwnerDavid Michael Honeinventor
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Abstract
A recombinant double stranded RNA (dsRNA) phage expresses dsRNA-encoded genes in eukaryote cells. Recombinant dsRNA phage are useful for the expression of dsRNA expression cassettes encoding passenger genes, such as, but not restricted to, vaccine antigens, bioactive proteins, immunoregulatory proteins, antisense RNAs, and catalytic RNAs in eukaryotic cells or tissues. Methods are provided to deliver recombinant dsRNA phage to eukaryotic cells and tissues, either by direct administration, formulated in lipid or polylactide-coglycolide, or by utilizing a bacterial vaccine vector.
Claims20
A double stranded RNA (dsRNA) phage that expresses at least one genetic sequence in eukaryote cells, comprising:
- a cap independent translation enhancer (CITE);
- and
- at least one genetic sequence that is expressed in a eukaryote cell, wherein said CITE and said at least one genetic sequence are functionally linked and are incorporated into one or more dsRNA segments in the dsRNA.
Dependencies are inferred from the claim text.