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US 7,018,805 B2Granted
α-Amylase assay and uses thereof
AssigneeVicam, L.P.
InventorBarb A. Cohen
Life of this patent
Estimating the term…
Abstract
This invention relates to methods and kits for measuring α-amylase activity in grain and plant products such as flour or stock.
Claims25
A method for measuring α-amylase activity in a flour or stock sample, comprising
- forming a reaction mixture by contacting a flour or stock sample with a detectably labeled starch substrate, for a time sufficient for α-amylase in the sample to hydrolyze the starch substrate, thereby releasing soluble detectably labeled starch fragments,
- separating the soluble detectably labeled starch fragments from the reaction mixture, wherein separating comprises contacting the fragments with an agent that binds to the detectably labeled starch fragments, and
- determining the level of hydrolysis of the detectably labeled starch substrate as a measurement of α-amylase activity in the flour or stock sample.
A method for measuring α-amylase activity in a flour or stock sample, comprising
- forming a reaction mixture by contacting a flour or stock sample with a detectably labeled starch substrate attached to a surface, for a time sufficient for α-amylase in the flour sample to hydrolyze the starch substrate, thereby releasing soluble detectably labeled starch fragments,
- separating the soluble detectably labeled starch fragments from the reaction mixture, wherein separating comprises contacting the fragments with an agent that binds to the detectably labeled starch fragments, and
- determining the level of hydrolysis of the detectably labeled starch substrate as a measurement of α-amylase activity in the flour or stock sample.
Dependencies are inferred from the claim text.