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US 7,018,803 B2Granted
Inhibitors of collagen assembly
AssigneeDrexel University College of Medicine
InventorsDarwin J. Prockop, Andrzej Fertala
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Abstract
The invention relates to peptides and peptidomimetics which inhibit assembly of human type I collagen. Methods of identifying such peptides and peptidomimetics are also included in the invention.
Claims11
A method of identifying a type I collagen assembly-inhibiting peptide, said method comprising
- immobilizing a collagen species on a support, wherein said collagen species is selected from the group consisting of human type I collagen and a human type I collagen peptide;
- contacting said support with a labeled peptide in the presence or absence of an unlabeled test peptide, wherein said labeled peptide is know to inhibit collagen self-assembly;
- and
- assessing the amount of labeled peptide bound to said support, wherein a lower amount of labeled peptide bound to said support in the presence of said test peptide compared with the level of binding of said labeled peptide to said support in the absence of said test peptide is an indication that said test peptide is a type I collagen assembly-inhibiting peptide.
1 more element
A method of identifying a type I collagen assembly-inhibiting peptide, said method comprising
- immobilizing collagen species on a support, wherein said collagen species is selected from the group consisting of human type I collagen and a human type I collagen peptide;
- contacting said support with at least one peptide-bearing particle which comprises a test peptide;
- and
- assessing whether said peptide-bearing particle binds with said support, whereby binding of said peptide-bearing particle to said support is an indication that said test peptide is a type I collagen assembly-inhibiting peptide.
1 more element
Dependencies are inferred from the claim text.