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US 7,018,802 B2Granted
Electrochemiluminescent enzyme immunoassay
AssigneeBioVeris Corporation
InventorsMark T. Martin, Rick Saul, Pam Liang
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AssigneeBioVeris Corporation
InventorsMark T. Martin, Rick Saul, Pam Liang
Estimating the term…
Electrochemiluminescent-labels and enzyme substrates, which preferably are conjugated, are used in immunoassays and electrochemiluminescence is generated catalytically. In conventional electrochemiluminescence immunoassays, an anti-analyte antibody molecule can give rise to typically 6–8 electrochemiluminescence-active ruthenium atoms, while in the present invention, each enzyme-labeled anti-analyte molecule can give rise to thousands of electrochemiluminescence-active ruthenium atoms per second. An exemplary immunoassay is based on a catalytic process employing β-lactamase-conjugated anti-analytes which enzymatically hydrolyze electrochemiluminescent-labeled substrates, making them strongly electrochemiluminescent. The electrochemiluminescence signal generated by each anti-analyte molecule (i.e., each analyte molecule) is much greater than with the conventional method. Accordingly, greater sensitivity can be gained in the measurement of low concentrations of a given immunoassay analyte.
A kit for measuring an analyte, comprising premeasured amounts of enzyme-conjugated anti-analyte, premeasured amounts of an electrochemiluminescent (ECL) label, a substrate that is converted into a product by the enzyme, and a reference standard, wherein a combination of the substrate and the ECL label generates less ECL than a combination of the product and the ECL label when an electrochemical potential is applied and wherein the premeasured amounts are sufficient to perform a single sample measurement.
A kit for measuring an analyte in a sample which comprises predetermined quantitative amounts of:
A kit for measuring an analyte, comprising premeasured amounts of enzyme-conjugated anti-analyte, premeasured amounts of an electrochemiluminescent (ECL) label, a substrate that is converted into a product by the enzyme, and a reference standard, wherein a combination of the substrate and the ECL label generates more ECL than a combination of the product and the ECL label when an electrochemical potential is applied and wherein the premeasured amounts are sufficient to perform a single sample measurement.
A kit for measuring an analyte in a sample which comprises predetermined quantitative amounts of:
Dependencies are inferred from the claim text.