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US 7,018,801 B2Granted
Selection of peptides with antibody-like properties
AssigneeBoard of Regents, The University of Texas System
InventorThomas J. Kodadek
Life of this patent
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Abstract
The present invention provides a highly sensitive screening assay for the identification of peptide binding partners to virtually any peptide or polypeptide ligand. Utilizing an expression-repression readout system, the inventors have screened libraries of peptides and identified relatively small peptide molecules that bind to the provided target.
Claims27
A method for identifying a peptide-peptide interaction comprising:
- (a) providing a first fusion construct comprising target peptide of 8 to 15 residues fused to a first DNA binding domain (DBD);
- (b) providing a second fusion construct comprising a library encoded peptide (LEP) of 5 to 50 residues fused to second DBD, wherein said second DBD works as a complex with said first DBD to facilitate binding of said complex to a prokaryotic operator region;
- (c) contacting said first and second fusion constructs in a prokaryotic host cell which comprises said prokaryotic operator region, wherein said prokaryotic operator region is operationally linked to a coding region for one or more indicator polypeptides;
- and
- (d) determining binding of said complex to said operator region,
- whereby binding of said complex to said operator region identifies said LEP as a binding partner for said target peptide.
3 more elements
Dependencies are inferred from the claim text.