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US 7,018,799 B2Granted
Methods for identifying inhibitors of neuronal degeneration
AssigneeScios, Inc.
InventorsJustin McCarthy, Barbara Cordell
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Abstract
Disclosed herein are methods for identifying inhibitors of neuronal degeneration, and their use in the treatment of neurodegenerative disorders. In particular methods methods are disclosed for identifying inhibitors of neuronal degeneration or cell death by taking advantage of the involvement of presenilin (PS) and Par-4 in NF-κB activation.
Claims24
A method for identifying an inhibitor of neuronal degeneration comprising:
- (a) providing eukaryotic host cells expressing a presenilin protein (PS), a prostate apoptosis response-4 (Par-4) polypeptide, and an NF-κB dependent reporter construct;
- (b) exposing the host cells to a candidate molecule;
- (c) monitoring the ability of said candidate molecule to induce NF-κB activation;
- (d) comparing the level of NF-κB activation in the host cells exposed to the candidate molecule to the level of the NF-κB activation in a control sample;
- and
- (e) identifying the inhibitor of neuronal degeneration when the level of NF-κB activation in the exposed cells is significantly greater than the level of NF-κB activation in the control sample.
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Dependencies are inferred from the claim text.