Binding complexes
AssigneesThe Institute of Cancer Research: Royal Cancer Hospital, Cancer Research Campaign Technology Limited
InventorsKeith Willison, Gillian Hynes, Anthony Kian-Fong Liou
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Abstract
The present invention relates to binding members that are capable of binding to and effecting the function of proteins useful in facilitating folding of large polypeptides. The present invention particularly relates to the chaperone CCT. The invention provides materials and methods for effecting the biological activity of CCT within the cell so as to prevent the folding to CCT substrates such as actin, tubulin or cyclin. The inventors provide specific binding members capable of occupying a CCT substrate binding site thereby preventing the substrate from binding. Further, the present invention provides methods for screening for such binding members which effect the biological activity of CCT.
Claims18
A method for identifying a binding member capable of occupying a substrate binding site on the CCT complex or part thereof, wherein the binding member inhibits the binding of the CCT substrate to the CCT complex or part thereof.
A method of identifying a binding member capable of occupying a substrate binding site on a CCT apical domain;
- comprising the steps of
- contacting a candidate binding member with said CCT apical domain;
- and
- determining binding between said candidate binding member and the CCT apical domain
- wherein the binding member inhibits the binding of the CCT substrate to the CCT apical domain.
Dependencies are inferred from the claim text.