US 7,015,205 B1 · Melanoma vaccine and methods of making and using same

Abstract

An immunotherapeutic vaccine providing antigen presenting cells that have been pulsed with a disrupted cell preparation which includes enucleated cytosol and cell membranes of cancer cells infected with a recombinant vaccinia virus encoding at least one immunostimulating molecule. In a preferred embodiment, the vaccine includes autologous dendritic/monocytic cells (DC/M) that present a mixture of antigens (present in the enucleated cytosol and cell membranes) from melanoma cell lines that have been infected with a recombinant vaccinia virus encoding IL-2. In another of the preferred embodiments, the enucleated cytosol and cell membranes are from melanoma cells harvested from the patient to be treated. A method of making the vaccine and methods of using the vaccine to stimulate an anti-cancer immune response and to treat a patient with a cancer are also described.

Claims38

Dependent claims

A method for eliciting an anti-cancer immune response in a subject, which comprises:

(a) administering a first recombinant vaccinia virus encoding at least one first immunostimulating molecule, wherein the first immunostimulating molecule is IL-2;
and
(b) administering a composition comprising antigen presenting cells, which are capable of inducing T cell activation, wherein the antigen presenting cells are dendritic cells and/or monocytes, and which are autologous or syngeneic, pulsed with a preparation comprising enucleated cytosol and cell membranes of cancer cells, which are derived from the subject or are the same cancer cell type as the patient-derived cancer cells, infected with a second recombinant vaccinia virus encoding at least one second immunostimulating molecule, wherein the second immunostimulating molecule is IL-2;
and
(c) wherein administration of said first recombinant vaccinia virus and said composition is at or near lymph node(s);
and wherein administration of said first recombinant vaccinia virus is approximately 30 minutes prior to said composition.

3 more elements

Dependencies are inferred from the claim text.

Melanoma vaccine and methods of making and using same

Life of this patent

Melanoma vaccine and methods of making and using same

Life of this patent

Abstract

Claims38