# US 7,015,033 B1 · Co-expression of recombination proteins

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,015,033 B1
- Document ID: US7015033B1
- Status: Granted
- Kind: B1
- Application number: 09/577,601
- Filed: 2000-05-25
- Granted: 2006-03-21
- Earliest claimed: 2000-05-25
- Assignees: Aventis Pasteur Limited
- Inventors: Sheena M. Loosmore; Yan-Ping Yang
- Web page: https://patentgrep.com/patent/US7015033B1

## CPC

- C07K 14/285: from Pasteurellaceae (F), e.g. Haemophilus influenza
- C12N 15/63: Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N 15/70: Vectors or expression systems specially adapted for E. coli
- C07K 2319/00: Fusion polypeptide
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Expression vectors are described which permit the recombinant expression of proteins which essentially contain, in addition to nucleic acid encoding the recombinant protein, nucleic acid encoding a non-proteolytic analog of Haemophilus Hin47 protein, with or without leader sequence, or nucleic acid encoding high molecular weight proteins of non-typeable Haemophilus, which are hmwB, hmwC or hmwBC.

## Claims

### Claim 1

An expression vector for expression of a recombinant protein in a host cell, comprising:
a nucleic acid molecule encoding a non-proteolytic mutant of a Haemophilus Hin47 protein wherein said Hin47 protein is mutated at amino acid 91, 121 or 197 and said nucleic acid molecule includes a portion encoding the leader sequence for said non-proteolytic mutant,
at least one additional nucleic acid molecule encoding the recombinant protein, wherein the recombinant protein is a Hia protein of a strain of Haemophilus influenzae which is N-terminally truncated, and
at least one regulatory element operatively connected to said first nucleic acid molecule and at least one regulatory element operatively connected to said at least one additional nucleic acid molecule to effect expression of at least said recombinant protein in the host cell.

### Claim 2 (depends on 1)

The vector of claim 1 wherein histidine 91 is replaced by alanine.

### Claim 3 (depends on 2)

The vector of claim 2, wherein said N-terminal truncation is V38.

### Claim 4 (depends on 2)

The vector of claim 2, wherein said N-terminal truncation is S44.