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US 7,015,030 B1Granted
Microfluidic devices and uses thereof in biochemical processes
AssigneesGenset S.A., Commissariat a l'Energie Atomique
InventorsYves Fouillet, Claude Vauchier, Jean-Frederic Clerc, Christine Peponnet
Life of this patent
Estimating the term…
Abstract
Provided is a microfluidic device comprising a microfluidic substrate comprising at least one pathway for sample flow; and at least one thermal transfer member which is capable of cycling between at least two temperatures. The thermal transfer member is adapted to heat at least a portion of the sample pathway while a sample is flowing along said at least a portion of said sample pathway. Provided also are methods of carrying out biochemical protocols using such a device.
Claims40
A device comprising:
- a microfluidic substrate comprising at least one sample pathway for sample flow;
- and
- said microfluidic substrate further comprising at least one temperature regulated zone that cycles between at least two different and predetermined temperatures, said at least one temperature regulated zone being adapted to bring at least a portion of said sample pathway to said at least two temperatures while a sample is unidirectionally flowing along said at least a portion of said sample pathway and said at least one temperature regulated zone comprising a metal bar in fluid communication with a plurality of water sources containing water at said at least two temperatures, said metal bar being in thermal communication with said at least a portion of said sample pathway and wherein a sample is cycled between said at least two different and predetermined temperatures while in said at least one temperature regulated zone.
Dependencies are inferred from the claim text.