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US 7,013,219 B2Granted
Regulation of endogenous gene expression in cells using zinc finger proteins
AssigneeSangamo BioSciences, Inc.
InventorsCasey C. Case, George N. Cox, III, Stephen P. Eisenberg, Qiang Liu, Edward J. Rebar
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Abstract
The present invention provides methods for modulating expression of endogenous cellular genes using engineered zinc finger proteins.
Claims22
A method for modulating expression of an endogenous gene in a cell, wherein the method comprises:
- a) administering to the cell a nucleic acid molecule comprising a polynucleotide sequence encoding an engineered zinc finger protein, wherein the engineered zinc finger protein comprises a plurality of zinc fingers and further wherein the amino acid sequence of at least one of the zinc fingers is obtained from the information in a database of existing zinc finger proteins and their respective binding affinities;
- and
- b) maintaining the cell under conditions in which the engineered zinc finger protein is expressed and binds to a target site in the gene.
A method for modulating expression of an endogenous gene in a cell, wherein the method comprises:
- a) administering to the cell a nucleic acid molecule comprising a polynucleotide sequence encoding an engineered zinc finger protein, wherein the engineered zinc finger protein comprises first and second zinc fingers and further wherein
- (i) the first zinc finger is obtained from a first zinc finger protein, and
- (ii) the second zinc finger is obtained from a second zinc finger protein that is different from the first zinc finger protein;
- and
- b) maintaining the cell under conditions in which the engineered zinc finger protein is expressed and binds to a target site in the gene.
2 more elements
Dependencies are inferred from the claim text.