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AssigneeUniversite Libre de Bruxelles
InventorsFrank Dubois, Catherine Yourassowski
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A method and device for obtaining a sample with three-dimensional microscopy, in particular a thick biological sample and the fluorescence field emitted by the sample. One embodiment includes obtaining interferometric signals of a specimen, obtaining fluorescence signals emanating from the specimen, recording these signals, and processing these signals so as to reconstruct three-dimensional images of the specimen and of the field of fluorescence emitted by the specimen at a given time. Another embodiment includes a digital holography microscope, a fluorescence excitation source illuminating a specimen, where the microscope and the fluorescence excitation source cooperate to obtain interferometric signals of the specimen and obtain fluorescence signals emanating from the specimen, means for recording the interferometric signals and fluorescence signals, and means for processing the interferometric signals and the fluorescence signals so as to reconstruct three-dimensional images of the specimen and of the field of fluorescence emitted by the specimen at a given time.
A method of obtaining three-dimensional images of a specimen and of a field of fluorescence emitted by the specimen, the method comprising, with a system able to operate in digital holography microscopy:
A system for obtaining three-dimensional images of a specimen and of a field of fluorescence of the specimen, wherein
A method of obtaining, by digital holography microscopy, three-dimensional images of a specimen and of a field of fluorescence emitted by the specimen, the method comprising:
A method of obtaining, by digital holography microscopy, three-dimensional images of a specimen and of a field of fluorescence emitted by the specimen, the method comprising:
A method of obtaining, by digital holography microscopy, three-dimensional images of a specimen and of a field of fluorescence emitted by the specimen, the method comprising:
A system for obtaining three-dimensional images of a specimen and of a field of fluorescence of the specimen, wherein the system simultaneously obtains digital holographic information of the entire volume of the specimen, simultaneously obtains fluorescence signals emanating from the entire volume of the specimen, records the digital holographic information and the fluorescence signals, processes the digital holographic information so as to reconstruct a three-dimensional image of the entire volume of the specimen at a given time, and processes the fluorescence signals, including combining the fluorescence signals with the digital holographic information, so as to reconstruct a three-dimensional image of the field of fluorescence emitted by the entire volume of the specimen at a given time, wherein the system comprises:
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