Method for investigating a sample
AssigneeCarl Zeiss Jena GmbH
InventorsRalf Wolleschensky, Bernhard Zimmermann, Sesbastian Tille
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Abstract
A method for investigating specimens, wherein a spectral splitting of the radiation coming from the specimen is carried out for specimen points or point distributions, for the operation of a laser scanning microscope or a fluorescence screening arrangement or a flow cylinder comprising the steps of generating a λ-stack so that the spectral distribution is measured by individual detection channels and storing the signals so as to be correlated to the detection signals with at least one of the spatial coordinates x, y and z and/or so as to be correlated to the measurement time t.
Claims29
A method for investigating specimens for the operation of a laser scanning microscope or a fluorescence screening arrangement or a flow cytometer, the method comprising the steps of:
- spectrally splitting a radiation coming from the specimen for specimen points or point distributions;
- generating a λ-stack from the spectrally split radiation, which includes:
- measuring the spectral distribution of the spectrally split radiation by individual detection channels of a detector;
- and
- storing signals correlated to detection signals of the detection channels with at least one of spatial coordinates x, y or z and/or correlated to a measurement time t.
Dependencies are inferred from the claim text.