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AssigneeArcher Daniels Midland Company
InventorsCharles Abbas, Andrii Y. Voronovsky, Liubov R. Fayura, Barbara V. Kshanovska, Kostiantyn V. Dmytruk, Kateryna A. Sibirna, Andrii A. Sibirny
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This invention is directed to the transformation of the flavinogenic yeasts, Pichia guilliermondii and Candida famata, and mutants thereof, by electroporation (electrotransformation) and by spheroplast transformation. The invention is also directed to nucleic acid constructs such as vectors, plasmids, and ARS sequences which transform flavinogenic yeasts, and mutants thereof, at a high level and in a stable manner so as to result in stably transformed yeast host cells which express/produce recombinant products. This invention also is directed to flavinogenic yeasts, Pichia guilliermondii and Candida famata, and mutants and temperature sensitive mutants thereof, which produce or overproduce riboflavin.
An isolated polynucleotide molecule comprising an ARS nucleotide sequence, having the function of enhancing the transformation efficiency and the maintenance of vectors as stable extrachromosomal elements in Candida famata;
A method for the transformation of Candida famata cells comprising electroporating a cell suspension containing said cells together with one or more nucleic acid constructs comprising one or more regulatory sequences and one or more genes or gene segments using one or more of resistance, field strength and pulse duration sufficient to transform said cells, wherein said field strength is from about 8 to about 15 kV/cm.
Dependencies are inferred from the claim text.