Method of measurement of nucleated red blood cells
AssigneeBeckman Coulter, Inc.
InventorsLidice L. Lopez, Carlos A. Perez, Mark A. Wells, Diana B. Careaga, Ziling Huo, Cheng Qian
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Abstract
Methods are provided for measurement of nucleated red blood cells. The methods include exposing a blood cell sample to a reagent system to lyse mature red blood cells, subsequently analyzing nucleated red blood cells in a flow cell by axial light loss, low angle light scatter and DC impedance measurements, or two selected measurements thereof; and then differentiating nucleated red blood cells from other cell types by using measured signals and/or functions thereof.
Claims26
A method for differentiating nucleated red blood cells in a blood cell sample comprising the steps of;
- (a) exposing said blood cell sample to a reagent system to lyse mature red blood cells and to form a sample mixture;
- (b) analyzing said sample mixture in a flow cell by measuring axial light loss signals and DC impedance signals;
- (c) differentiating nucleated red blood cells from other cell types using signals consisting of said axial light loss and DC impedance signals obtained in step (b);
- and
- (d) reporting nucleated red blood cells in said blood cell sample.
A method for differentiating nucleated red blood cells in a blood cell sample comprising the steps of:
- (a) exposing said blood cell sample to a reagent system to lyse mature red blood cells and to form a sample mixture;
- (b) analyzing said sample mixture in a flow cell by measuring low angle light scalier signals and an axial light loss signals;
- (c) differentiating nucleated red blood cells from other cell types using signals consisting of said low angle light scalier and axial light loss signals obtained in step (b);
- and
- (d) reporting nucleated red blood cells in said blood cell sample.
A method for differentiating nucleated red blood cells in a blood cell sample comprising the steps of:
- (a) exposing said blood cell sample to a reagent system to lyse mature red blood cells and to form a sample mixture;
- (b) analyzing said sample mixture in a flow cell by measuring DC impedance signals, low angle light scatter signals and axial light loss signals;
- (c) differentiating nucleated red blood cells from other cell types using signals consisting of said DC impedance, low angle light scatter and axial light loss signals obtained in step (b);
- and
- (d) reporting nucleated red blood cells in said blood cell sample.
Dependencies are inferred from the claim text.