US 7,008,784 B1Granted

Non-infectious, non-replicating, immunogenic human immunodeficiency virus-like particles

AssigneeAventis Pasteur Limited

InventorsJoel Haynes, Michel Henri Klein, Benjamin Rovinski, Shi Xian Cao

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Abstract

The present invention is directed toward methods for the production of non-infectious, replication-deficient, immunogenic human immunodeficiency virus (HIV)-like particles. These particles are prepared from a recombinant expression vector comprising a heterologous promoter operatively connected to a DNA molecule comprising a modified HIV genome devoid of the long terminal repeat (LTR) regulatory regions but containing at least the gag and pol genes in their natural genomic arrangement. This vector is introduced into mammalian cells to produce the particles of interest. These particles should prove useful in a number of diagnostic, virologic, and immunologic applications.

Claims5

Dependent claims

A method for obtaining a non-infectious, non-replicating and immunogenic human immunodeficiency virus (HIV)-like particle comprising at least the HIV gag and pol gene products wherein said method comprises the following steps:

a) preparing a recombinant expression vector comprising a heterologous promoter operatively connected to a DNA molecule comprising a DNA sequence comprising a modified HIV genome devoid of the long terminal repeats (LTRs) but containing at least the gag and pol genes in their natural genomic arrangement,
b) introducing the expression vector into mammalian cells,
c) expressing said DNA sequence in said mammalian cells to produce non-infectious, non-replicating and immunogenic human immunodeficiency virus (HIV)-like particles comprising at least the HIV gag and pol gene products.

Dependencies are inferred from the claim text.

Facts

Application: 09/389,364Filed 3 Sep 1999 · division
Priority: GB 892312313 Oct 1998
Granted: 7 Mar 2006
Term ends: Not estimatedThe source records don’t support an estimate.

Classification19 codes

C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
A61P 31/12
Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics: antivirals
A61K 39/00
Medicinal preparations containing antigens or antibodies
C12N 2740/15023
Lentivirus, not HIV, e.g. FIV, SIV: virus like particles [VLP]

Family

Application07/829,751Parent of this continuation · filed 15 Jun 1992
Application08/401,355Parent of this division · filed 9 Mar 1995
US 6,291,227 B1Parent of this division
US 5,439,809 AParent of this continuation
Application08/401,355Parent of this continuation
US 5,258,198 ARelated patent
US 7,008,784 B1This patent

From the US filing history. Foreign family members aren’t listed.