Gene encoding chondroitinase ABC and uses therefor
AssigneeMaruha Corporation
InventorsNobuyuki Sato, Masahiko Shimada, Hiroshi Oda
Life of this patent
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Abstract
Nucleic acid sequences coding for the chondroitinase ABC gene and isolated chondroitinase ABE protein produced in a host cell transformed with a nucleic acid vector directing the expression of a nucleotide sequence coding for chondroitinase ABE protein described. Chondroitinase ABC prepared by chemical synthesis also described. Monoclonal and polyclonal antibodies which are specifically reactive with chondroitinase ABC protein are disclosed. The isolated chondroitinase ABC can be used in methods of treating intervertebral disc replacement, promoting neurite regeneration, and detecting galactosaminoglycans.
Claims28
An isolated nucleic acid fragment encoding chondroitinase ABC, comprising the nucleotide sequence of SEQ ID NO:
- 1.
An isolated nucleic acid encoding chondroitinase ABC comprising a nucleotide sequence which differs from the nucleotide sequence of SEQ ID NO:
- 1, due to degeneracy in the genetic code.
An isolated nucleic acid fragment comprising the coding region of chondroitinase ABC and having a nucleotide sequence consisting of nucleotides 297–3288 of SEQ ID NO:
- 1.
An isolated nucleic acid comprising a nucleotide sequence which differs from nucleotides 297–3288 of SEQ ID NO:
- 1, due to degeneracy in the genetic code.
An isolated nucleic acid fragment comprising nucleotides 2160–3288 of SEQ ID NO:
- 1.
An isolated nucleic acid comprising a nucleotide sequence which differs from nucleotides 2160–3288 of SEQ ID NO:
- 1, due to degeneracy in the genetic code.
An expression vector comprising the nucleic acid as defined in claims operably linked to a regulatory sequence.
An isolated nucleic acid fragment comprising the coding region of the nucleotide sequence of SEQ ID NO:
- 1.
An isolated nucleic acid comprising a nucleotide sequence which differs from the coding region of SEQ ID NO:
- 1, due to degeneracy in the genetic code.
Dependencies are inferred from the claim text.