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US 7,008,773 B1Granted
Method for identifying active anti apoptosis compounds
AssigneeCytoTools GmbH
InventorsMark A. Freyberg, Peter Friedl, Dirk Kaiser
Life of this patent
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Abstract
The invention relates to a method for identifying substances with antiapoptotic activity, where i) cells which express both IAP and the integrin αvβ3 are cultured, ii) the cells are caused to produce an apoptosis-inducing substance, and/or a substance or substances which induces/induce apoptosis is/are added, iii) the test substance is added, and iv) the apoptosis rate is measured, and to the substances which can be identified with the method.
Claims6
A method for identifying test substances with antiapoptotic activity, comprising
- i) culturing cells selected from the group consisting of endothelial cells, smooth muscle cells, and fibroblasts which express both CD47 and the integrin αvβ3,
- ii) causing the cells to produce an apoptosis-inducing substance by culturing under static or turbulent flow conditions, and/or adding a substance which simultaneously binds CD47 and αvβ3, thereby inducing apoptosis,
- iii) adding a test substance, and
- iv) determining a change in apoptosis rate by determining the portion of cells with apoptotic nuclei by one of DAPI staining, tunnel assay, DNA ladder, annexin staining, or measuring increased calcium influx into the cells,
- wherein a decrease in apoptosis rate in step iy) is indicative of the test substance having antiapoptotic activity.
2 more elements
Dependencies are inferred from the claim text.