Method for detecting radiation exposure

A method of identifying cells that have been exposed to radiation induced biological stress, the method comprising:

• providing a plurality of probe elements bound to a surface, each probe element comprising a nucleic acid that is differentially expressed by a cell following ionizing radiation induced biological stress, wherein at least one probe element comprises SEQ ID NO:
• 1;
• contacting the plurality of probe elements with a plurality of test nucleic acid sequences obtained from a test cell, under conditions that allow the test nucleic acid sequences to specifically hybridize to one or more of the probe elements, and provide a signal which indicates differential expression of one or more genes if the cell has been exposed to an amount of ionizing radiation sufficient to cause differential expression of genes in the cell;
• and
• detecting the presence or absence of the signal.

A method of making a microarray for identifying cells that have actually or potentially been exposed to an amount of ionizing radiation sufficient to cause differential expression of genes in the cell, comprising:

• identifying genes that are differentially expressed by a cell following exposure to the amount of ionizing radiation sufficient to cause differential expression of genes in the cell;
• and
• providing a plurality of probe elements bound to a substrate surface, the probe elements comprising probe nucleic acids from genes that are identified as differentially expressed by a cell following ionizing radiation induced biological stress, wherein at least one probe element comprises SEQ ID NO:
• 1, and wherein the probe nucleic acid is capable of hybridizing to a nucleic acid differentially expressed by the cell following exposure of the cell to the ionizing radiation.

A method of diagnosing ionizing radiation exposure in a subject, comprising:

• obtaining a biological sample from the subject;
• synthesizing cDNA from mRNA expressed in one or more cells of the biological sample, and labeling the cDNA with a detectable label;
• exposing the labeled cDNA to a probe set which represents genes that are differentially expressed in the biological sample following exposure to ionizing radiation, and determining if the labeled cDNA selectively hybridizes to one or more probes of the probe set that are associated with the radiation exposure, wherein the probe set comprises SEQ ID NO:
• 1.

A method for measuring a biological response in a subject comprising:

• obtaining a biological sample from the subject;
• synthesizing cDNA from mRNA expressed in one or more cells of the biological sample;
• labeling the cDNA with a detectable label;
• exposing the labeled cDNA to a probe set comprising nucleic acid molecules representing genes that are differentially expressed in cells that have been exposed to an amount of ionizing radiation sufficient to cause differential expression of genes in the cell, wherein at least one nucleic acid molecule of the probe set comprises SEQ ID NO:
• 1;
• and
• determining if the labeled cDNA selectively hybridizes to one or more probes of the probe set differentially expressed following exposure to ionizing radiation.

A method of identifying cells that have been exposed to radiation induced biological stress, the method comprising:

• contacting a plurality of probe elements with a plurality of test nucleic acid sequences obtained from a test cell, under conditions that allow the test nucleic acid sequences to specifically hybridize to one or more of said plurality of probe elements, and provide a signal which indicates differential expression of one or more genes if the cell has been exposed to an amount of ionizing radiation sufficient to cause differential expression of genes in the cell, wherein the plurality of probe elements comprise nucleic acid sequences that are differentially expressed by a cell following ionization radiation induced biological stress, wherein at least one probe element of said plurality of probe elements comprises SEQ ID NO:
• 1;
• and
• detecting the presence or absence of the signal.