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US 7,008,766 B1Granted
Nucleic acid sequence analysis
AssigneeMedical Biosystems, Ltd.
InventorDaniel Henry Densham
Life of this patent
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Abstract
The present invention relates to a method for determining the sequence of a polynucleotide, the method comprising the steps of: (i) reacting a target polynucleotide with a polymerase enzyme immobilised on a solid support, and the different nucleotides, under conditions sufficient for the polymerase reaction; and (ii) detecting the incorporation of a specific nucleotide complementary to the target polynucleotide, by measuring radiation.
Claims24
A method for sequencing a polynucleotide, comprising the steps of:
- (i) reacting a target polynucleotide with a polymerase, wherein said polymerase is immobilised on a solid support, and nucleotides, under conditions sufficient for the polymerase reaction;
- and
- (ii) detecting the interaction between the polymerase, the target polynucleotide and each specific nucleotide complementary to the target polynucleotide, that is incorporated into a nascent polynucleotide being synthesized as a result of the polymerase reaction, to thereby determine the sequence of the target polynucleotide, the detection being carried out by using surface plasmon resonance to measure a change in, or absorption of, radiation that occurs during the interaction.
Dependencies are inferred from the claim text.