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US 7,002,060 B1Granted
Enzymes involved in petroselinic acid biosynthesis
AssigneeE.I. du Pont de Nemours and Company
InventorEdgar B. Cahoon
Life of this patent
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a gene involved in petroselinic acid biosynthesis. The invention also relates to the construction of a chimeric gene encoding all or a portion of the gene involved in petroselinic acid biosynthesis, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the gene involved in petroselinic acid biosynthesis in a transformed host cell.
Claims15
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having Δ4-16:0-ACP desaturase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2 have at least 95% sequence identity based on the Clustal alignment method, or
- (b) the complement of the nucleotide sequence of (a).
An isolated nucleic acid molecule comprising:
- (a) at least 300 nucleotides and
- (b) said isolated nucleic acid molecule remains hybridized to an isolated polynucleotide comprising:
- (i) a nucleotide sequence encoding a polypeptide having Δ4-16:0-ACP desaturase activity, said nucleotide sequence comprising the nucleotide sequence of SEQ ID NO:1, or
- (ii) the complement of the nucleotide sequence of (i), under a wash condition of 0.1×SSC, 0.1% SDS, and 65° C.
1 more element
Dependencies are inferred from the claim text.