Soybean transformation method
AssigneeMonsanto Technology LLC
InventorsBrian J. Martinell, Lori S. Julson, Carol A. Emler, Yong Huang, Dennis E. McCabe, Edward J. Williams
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Abstract
A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.
Claims12
A method of making germline-transformed soybean plants using Agrobacterium mediation, the method comprising:
- (a) initiating the germination of a soybean seed;
- (b) isolating the embryonic axis including the embryonic meristem from the soybean seed to prepare an explant;
- (c) exposing the explant to a disarmed Agrobacterium vector comprising a heterologous genetic construct comprising a selectable marker gene wherein the heterologous genetic construct is transferred into at least one cell in the explant;
- (d) culturing the explant in the presence of a selection agent in a manner allowing identification of soybean cells of the explant to which the heterologous genetic construct has been transferred;
- (e) inducing formation of one or more shoots from the explant, the shoot comprising germline transformed cells;
- (f) cultivating the shoot into a whole fertile mature soybean plant.
A method of transforming a soybean cell using Agrobacterium comprising:
- (a) initiating the germination of a soybean seed;
- (b) isolating the embryonic axis including the embryonic meristem from the soybean seed to prepare an explant;
- (c) exposing the explant to a disarmed Agrobacterium vector comprising a heterologous genetic construct comprising a selectable marker gene wherein the heterologous genetic construct is transferred into at least one cell in the explant;
- (d) culturing the explant in the presence of a selection agent in a manner allowing identification of soybean cells of the explant to which the heterologous genetic construct has been transferred.
Dependencies are inferred from the claim text.