Production of high molecular weight hyaluronates
AssigneeCalcigen Corporation
InventorMichael Berbaum
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Abstract
Methods of making high molecular weight hyaluronates are disclosed. The methods include contacting a hyaluronate-containing source with an acid to make an acidic hyaluronate suspension, contacting that suspension with an anionic ion exchange medium in the presence of an acidic buffer, and thereafter contacting the medium with an acidic buffer having a higher salt content to desorb the hyaluronate from the medium. The methods are amenable for isolating hyaluronate from such sources as avian (e.g., chicken, duck, turkey, or goose) eggshells and connective tissues of animals.
Claims20
A method of isolating high molecular weight hyaluronate from a hyaluronate-containing source, the method comprising
- contacting the source with an acidic solution to yield an acidic hyaluronate suspension,
- contacting the suspension with an anion exchange medium in the presence of an acidic, relatively low salt buffer, and
- thereafter contacting the medium with an acidic, relatively high salt buffer to obtain a second suspension comprising the isolated high molecular weight hyaluronate.
Dependencies are inferred from the claim text.