Synthetic human papillomavirus genes
AssigneeMerck & Co., Inc.
InventorsMichael P Neeper, William L. McClements, Kathrin U. Jansen, Loren D. Schultz, Ling Chen, Xin-Min Wang
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Abstract
Synthetic DNA molecules encoding papillomavirus proteins are provided. The codons of the synthetic molecules are codons preferred by the projected host cell. The synthetic molecules may be used as a polynucleotide vaccine which provides effective immunoprophylaxis against papillomavirus infection through stimulation of neutralizing antibody and cell-mediated immunity.
Claims15
A synthetic polynucleotide comprising a nucleotide sequence encoding a codon-optimized human papillomavirus serotype 16 (HPV16) protein, wherein said nucleotide sequence comprises codons that are optimized for expression in a human host, and wherein the protein is selected from the group consisting of:
- L1, E1, E2, and E7 of HPV16.
A synthetic polynucleotide comprising a nucleotide sequence encoding a codon-optimized human papillomavirus serotype 16 (HPV16) protein wherein said nucleotide sequence comprises codons that are optimized for expression in a human host, the polynucleotide comprising a sequence of nucleotides as set forth in SEQ.ID.NO:
- 1, wherein the polynucleotide is DNA.
A synthetic polynucleotide which comprises a sequence of nucleotides as set forth in SEQ.ID.NO:2.
A synthetic polynucleotide which comprises a sequence of nucleotides as set forth in SEQ. ID.NO:
- 3.
A synthetic polynucleotide which comprises a sequence of nucleotides as set forth in SEQ. ID.NO:4.
An adenoviral vaccine vector comprising an adenoviral genome with a deletion in the E1 region, and an insert in the E1 region, wherein the insert comprises an expression cassette comprising:
- A) a polynucleotide encoding a codon-optimized HPV16 protein selected from the group consisting of L1, E1, E2, and E7 proteins, wherein said polynucleotide is codon-optimized for expression in a human host cell;
- and
- B) a promoter operably linked to the polynucleotide.
A shuttle plasmid vector comprising a plasmid portion and an adenoviral portion, the adenoviral portion comprising:
- an adenoviral genome with a deletion in the E1 region, and an insert in the E1 region, wherein the insert comprises an expression cassette comprising:
- A) a polynucleotide encoding a codon-optimized HPV 16 protein selected from the group consisting of L1, E1, E2, and E7 proteins, wherein said polynucleotide is codon-optimized for expression in a human host cell;
- and
- B) a promoter operably linked to the polynucleotide.
A vaccine plasmid comprising a plasmid portion and an expression cassette portion, wherein the expression cassette portion comprises:
- A) a polynucleotide encoding a codon-optimized HPV16 protein selected from the group consisting of L1, E1, E2, and E7 proteins, wherein said polynucleotide is codon-optimized for expression in a human host cell;
- and
- B) a promoter operably linked to the polynucleotide.
Dependencies are inferred from the claim text.