Fluorescent proteins
AssigneeBioImage A/S
InventorsSara Petersen Bjorn, Len Pagliaro, Ole Thastrup
Life of this patent
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Abstract
A GFP with an F64L mutation and an E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan GFP allowing for cleaner band separation when used together with those GFPs.
Claims13
A fluorescent protein, as shown in SEQ ID NO:
- 4, wherein the amino acid in position 1 preceding the chromophore has been substituted by an aliphatic amino acid, and wherein the Glutamic acid in position 223 has been substituted by an amino acid selected from the group consisting of G, A, V. L and I.
A fluorescent protein, comprising a Green Fluorescent Protein (GFP) comprising an amino acid sequence wherein the amino acid at the position corresponding to position 65 of SEQ ID NO:4 is substituted with an aliphatic amino acid, and wherein the amino acid at the position corresponding to the position 223 of SEQ ID NO:4 has been substituted by an amino acid selected from the group consisting of G, A, V, L and I.
Dependencies are inferred from the claim text.