Genes encoding wheat starch synthases and uses thereof
AssigneesCommonwealth Scientific and Industrial Research Organisation, Biogemma, Goodman Fielder Pty Limited
InventorsMatthew Morell, Zhongyi Li, Sadequr Rahman, Rudolph Appels
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Abstract
The present invention provides isolated nucleic acid molecules encoding wheat starch syntheses, and probes and primers derived therefrom, which are useful in the modificaiton of plant starch content and/or composition, and for screening plant lines to determine the presence of natural and/or induced mutations in starch synthase genes which affect starch content and/or composition. More particularly the isolated nucleic acid molecules of the present invention further provide for the screening-assisted breeding of plants having desirable starch content and/or composition, in addition to providing for the direct genetic manipulation of plant starch content and/or composition.
Claims15
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having starch synthase II activity or a nucleotide sequence complementary thereto, said nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence having at least 97% identity to the nucleotide sequence of the protein-encoding region of the nucleotide sequence set forth in SEQ ID NO:
- 3;
- (ii) a nucleotide sequence encoding a polypeptide having at least 97% identity to the amino acid sequence set forth in SEQ ID NO:
- 4;
- and
- (iii) a nucleotide sequence which is complementary to (i) or (ii).
A plant comprising a transgenic nucleic acid molecule comprising a nucleotide sequence, said nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence encoding a polypeptide having starch synthase II activity having at least 97% identity to the nucleotide sequence of the protein-encoding region of the nucleotide sequence set forth in SEQ ID NO:
- 3;
- (ii) a nucleotide sequence encoding a polypeptide having starch synthase II activity the polypeptide having at least 97% identity to the amino acid sequence set forth in SEQ ID NO:
- 4;
- (iii) a nucleotide sequence which is complementary to (i) or (ii);
- and
- (iv) an antisense molecule or co-supression molecule which comprises a fragment of (i), (ii) or (iii) which is expressed to down-regulate the expression of an endogenous starch synthase isoenzyme of the plant.
Dependencies are inferred from the claim text.