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US 7,001,754 B2Granted
Methods and compositions for the introduction of molecules into cells
AssigneeStine Biotechnology
InventorsBruce Marvin Held, Herbert Martin Wilson, Liming Hou, Carol Jean Lewnau, Janelle Christine Eby
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Abstract
The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus production of elite lines of soybean.
Claims8
A method for the introduction of one or more nucleotide sequences into a cell, wherein the method comprises:
- (a) preparing a solution containing said nucleotide sequences;
- (b) supplying said solution for conversion to aerosol droplets which are produced by a microflow nebulizer at a flow rate of between about 1 μl/minute to about 100 μl/minute;
- (c) producing said aerosol droplets comprising said nucleotide sequences;
- (d) accelerating said aerosol droplets toward said cell;
- (e) impacting said cell with said accelerated aerosol droplets;
- and
- (f) producing a cell containing said nucleotide sequences.
4 more elements
Dependencies are inferred from the claim text.