PyrF gene and the utilization thereof
AssigneeConsortium fur Elektrochemische Industrie GmbH
InventorRupert Pfaller
Life of this patent
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Abstract
A pyrF gene is useful as a selection marker gene for an expression system for the production of proteins in mushrooms of the genus Trametes, Coriolus or Polyporus. The pyrF gene includes a DNA sequence SEQ ID NO: 1 from position 1133 up to and including position 1877 or DNA-sequence SEQ, ID NO: 2 from position 1 up to and including position 684 or a DNA-sequence with a sequence homology greater than 60% relative to the above-mentioned regions of sequence SEQ ID NO: 1 or SEQ ID NO: 2.
Claims5
An isolated DNA sequence which codes for a protein having an enzymatic activity of orotate phosphoribosyl-transferase (pyrF activity) which comprises:
- a DNA sequence selected from the group consisting of:
- the DNA sequence SEQ ID NO:
- 1 from position 1133 up to and including position 1877;
- and
- the DNA sequence SEQ ID NO:
- 2 in from position 1 up to and including position 684.
An isolated protein having pyrF activity, which comprises:
- an amino acid sequence SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.