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US 7,001,740 B2Granted
Methods of arraying biological materials using peelable and resealable devices
AssigneeSurface Logix, Inc.
InventorsDavid Duffy, Gregory L. Kirk, Stewart Campbell, Olivier Schueller, Melina Agosto
Life of this patent
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Abstract
Devices and methods for performing assays on materials, particularly biological materials, are provided. The devices and methods make use of self-sealing members, which can be applied to a flat surface to form wells to facilitate immobilization of materials on the flat surface, then removed to yield a flat surface that facilitates the performance of processes on and/or detection of the immobilized material.
Claims21
A method of arraying a biological material comprising:
- placing a removable member on a base plate for establishing self-sealing contact of the removable member on the base plate, the removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of orifices therein and having walls bounding respective ones of the plurality of orifices on sides of the orifices, the removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of wells therewith;
- immobilizing at least one biological material on regions of the base plate exposed by orifices of the removable member;
- removing the removable member from the base plate such that the biological material is arrayed on the base plate in an array that corresponds to an array defined by the orifices.
A method of arraying a biological material comprising:
- placing a first removable member on a base plate for establishing self-sealing contact of the first removable member on the base plate, the first removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of first orifices therein and having walls bounding respective ones of the plurality of first orifices on sides of the first orifices, the first removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of first wells therewith;
- immobilizing at least one biological material on regions of the base plate exposed by the first orifices;
- removing the first removable member from the base plate such that the biological material is arrayed on the base plate in an array that corresponds to an array defined by the first orifices;
- and
- placing a second removable member on the base plate for establishing self-sealing contact of the second removable member on the base plate, the second removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of second orifices therein and having walls bounding respective ones of the plurality of second orifices on sides of the orifices, the second removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of second wells therewith, the second wells being arrayed such that the biological material arrayed on the base plate is within the second wells.
2 more elements
A method of arraying a biological material comprising:
- placing a first removable member on a base plate for establishing self-sealing contact of the first removable member on the base plate, the first removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of first orifices therein and having walls bounding respective ones of the plurality of first orifices on sides of the first orifices, the first removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of first wells therewith;
- immobilizing at least one biological material on regions of the base plate exposed by the first orifices;
- placing a second removable member on the first removable member for establishing self-sealing contact of the second removable member on the first removable member, the second removable member being adapted to repeatedly self seal on the first removable member, further defining a plurality of second orifices therein and having walls bounding respective ones of the plurality of second orifices on sides of the orifices, the removable member further being configured such that, when it is placed in self-sealing contact with the first removable member, it defines a plurality of second wells therewith.
A method for arraying a biological material comprising:
- immobilizing at least one biological material on regions of a base plate, the regions defining an array;
- placing a removable member on the base plate for establishing self-sealing contact of the removable member on the base plate, the removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of orifices therein and having walls bounding respective ones of the plurality of orifices on sides of the orifices, the removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of wells therewith, the wells being arrayed such that the biological material arrayed on the base plate is within the wells.
Dependencies are inferred from the claim text.