Urinary trypsin inhibitor assay containing a chelating agent
AssigneeBayer Aktiengesellschaft
InventorsGary E. Rehm, Michael J. Pugia, Paul F. Corey
Life of this patent
Estimating the term…
Abstract
Disclosed is an assay for determining the presence and concentration of trypsin inhibitor in urine samples. The assay reagents, which may be used either in the liquid or dry states, include trypsin, a trypsin substrate and a polycarboxylic chelating agent. The inclusion of the chelating agent in the assay has been found to reduce variation in the assay results.
Claims11
An assay for trypsin inhibitors in urine which comprises (a) contacting a urine test sample with a buffered assay medium comprising (i) trypsin in an amount of from 10 to 750 IU/mL, (ii) a substrate for trypsin which will produce a detectable response when cleaved by trypsin present in a concentration of from 0.2 to 50 mM and (iii) a polycarboxylic chelating agent present in an amount of from 0.2 to 50 mM, and (b) correlating the concentration of trypsin inhibitor with the detectable response from the cleaving of the substrate, wherein the polycarboxylic chelating agent reduces variation in detected amounts of trypsin inhibitor caused by the presence of calcium ions in the urine in reference to a control sample lacking the polycarboxylic chelating agent.
Dependencies are inferred from the claim text.