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US 7,001,733 B1Granted
Methods and compositions for screening for modulations of IgE synthesis, secretion and switch rearrangement
AssigneeRigel Pharmaceuticals, Inc.
InventorsDavid A. Ferrick, Susan E. Swift, Randall Armstrong, Bryan Fox
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Abstract
The invention relates to methods and compositions useful in screening for modulators of IgE synthesis, secretion and switch rearrangement.
Claims21
A method of screening for a bioactive agent capable of inhibiting an IL-4 inducible ε promoter, said method comprising
- a) introducing a library of nucleic acid molecules encoding randomized peptide candidate bioactive agents into a population of cells, said cells comprising a fusion nucleic acid which fusion nucleic acid comprises:
- i) an IL-4 inducible ε promoter consisting of the sequence set forth as SEQ ID NO:1;
- and
- ii) a reporter gene;
- b) inducing said promoter with IL-4;
- and
- c) detecting the level of a reporter protein encoded by said reporter gene,
- wherein a reduction in the level of said reporter protein compared to the level of the reporter protein in the absence of the candidate bioactive agent indicates that said agent inhibits said IL-4 inducible ε promoter.
5 more elements
A method of screening for a bioactive agent capable of inhibiting an IL-4 inducible ε promoter, said method comprising:
- a) introducing a candidate bioactive agent into a cell, said cell comprising a fusion nucleic acid which fusion nucleic acid comprises:
- i) an IL-4 inducible ε promoter consisting of the sequence set forth as SEQ ID NO:1;
- and
- ii) a reporter gene;
- b) inducing said promoter with IL-4;
- and
- c) detecting the level of a reporter protein encoded by said reporter gene,
- wherein a reduction in the level of said reporter protein compared to the level of the reporter protein in the absence of the candidate bioactive agent indicates that said agent inhibits said IL-4 inducible ε promoter.
5 more elements
Dependencies are inferred from the claim text.