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US 7,001,719 B2Granted
Devices and methods for microorganism detection
Assignee3M Innovative Properties Company
InventorsJames H. Wicks, Carl A. Adams, Gary E. Krejcarek
Life of this patent
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Abstract
A device that includes at least two chambers separated by an activatable seal wherein upon activation of the seal the two chambers are in communication, and further wherein at least one chamber of the device includes a biological assay reagent and methods of using same.
Claims4
A method for detecting the presence or absence of bacteria, the method comprising:
- providing a device comprising at least three chambers separated from each other by seals, wherein a first chamber includes bacteriophage, a second chamber includes an antiviral agent, and a third chamber includes bacterial helper cells, wherein the second chamber is disposed between the first and third chambers;
- adding a sample suspected of including target bacteria to the first chamber comprising bacteriophage;
- allowing the bacteriophage to infect the target bacteria;
- activating the seal between the first and second chambers to allow contact between the antiviral agent and extracellular bacteriophage;
- activating the seal between the second and third chambers to allow contact between the bacterial helper cells and the infected target bacteria;
- incubating the bacterial helper cells and the infected bacteria;
- and
- detecting the presence or absence of the target bacteria in the sample.
5 more elements
Dependencies are inferred from the claim text.