Method of inhibiting pathogenicity of infectious agents
AssigneesMassachusetts Institute of Technology, Arizona Board of Regents
InventorsBertram L. Jacobs, Alexander Rich
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods of detecting or identifying inhibitors of a Z-DNA binding ligand to Z-DNA, methods of inhibiting the pathogenicity of an infectious agent, antiviral therapies, and compounds that inhibit complex formation between a Z-DNA binding ligand and Z-DNA.
Claims51
A method of detecting and/or identifying an inhibitor of binding of a Z-DNA binding ligand to Z-DNA, comprising the steps of:
- a) combining an agent to be tested, Z-DNA, and a composition comprising a Z-DNA binding ligand, under conditions suitable for binding of said Z-DNA binding ligand to said Z-DNA;
- and
- b) detecting the formation of a complex between said Z-DNA binding ligand and said Z-DNA, wherein inhibition of formation of said complex by said agent relative to a suitable control is indicative that the agent is an inhibitor,
- wherein said Z-DNA binding ligand is a fusion protein comprising a Z-DNA binding ligand and a detectable label, and wherein formation of said complex is detected by detecting said detectable label.
A method of detecting and/or identifying an anti-infective agent, comprising the steps of:
- a) combining an agent to be tested, Z-DNA and a composition comprising a Z-DNA binding ligand under conditions suitable for binding of said Z-DNA binding ligand to said Z-DNA;
- b) detecting the formation of a complex between said Z-DNA binding ligand and said Z-DNA, wherein inhibition of formation of said complex by said agent relative to a suitable control is indicative that the agent is an anti-infective agent;
- and
- c) assessing the anti-infective activity of said agent in a suitable assay.
A method of detecting and/or identifying an anti-infective agent, comprising the steps of:
- a) contacting a cell comprising a Z-DNA binding ligand and Z-DNA with an agent to be tested, under conditions suitable for binding of said Z-DNA binding ligand to said Z-DNA;
- and
- b) detecting the formation of a complex between said Z-DNA binding ligand and said Z-DNA, wherein inhibition of formation of said complex by said agent relative to a suitable control is indicative that the agent is an anti-infective agent;
- and
- c) assessing the anti-infective activity of said agent in a suitable assay.
A method of detecting and/or identifying an inhibitor of binding of a Z-DNA binding ligand to Z-DNA, comprising the steps of:
- a) combining an agent to be tested, a composition comprising a Z-DNA binding ligand and Z-DNA, under conditions suitable for binding of said Z-DNA binding ligand to said Z-DNA;
- and
- b) detecting the formation of a complex between said Z-DNA binding ligand and said Z-DNA, wherein inhibition of formation of said complex by said agent relative to a suitable control is indicative that the agent is an inhibitor,
- wherein said Z-DNA binding ligand is selected from the group consisting of E3L, ADAR1, DLM-1, and a fusion protein comprising any one of the foregoing.
A method of detecting and/or identifying an anti-viral agent, comprising the steps of:
- a) combining an agent to be tested, Z-DNA and a composition comprising a Z-DNA binding ligand, under conditions suitable for binding of said Z-DNA binding ligand to said Z-DNA;
- b) detecting the formation of a complex between said Z-DNA binding ligand and said Z-DNA, wherein inhibition of formation of said complex by said agent relative to a suitable control is indicative that the agent is an anti-viral agent;
- and
- c) assessing the anti-viral activity of said agent in a suitable assay.
A method of detecting and/or identifying an anti-poxvirus agent, comprising the steps of:
- a) combining an agent to be tested, Z-DNA and a composition comprising a protein, said protein comprising a poxvirus E3L or a Z-DNA binding domain thereof, under conditions suitable for binding of said poxvirus E3L or a Z-DNA binding domain thereof to said Z-DNA;
- and
- b) detecting the formation of a complex between said poxvirus E3L or a Z-DNA binding domain thereof and said Z-DNA, wherein inhibition of formation of said complex by said agent relative to a suitable control is indicative that the agent is an anti-poxvirus agent.
Dependencies are inferred from the claim text.