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US 7,001,716 B2Granted
Continuous flow methods for monitoring time dependent reactions
AssigneeCaliper Life Sciences, Inc.
InventorsSamuel D. H. Chan, Ring-Ling Chien, Andrea W. Chow, Benjamin N. Wang
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Abstract
Methods for monitoring time dependent reactions that comprise providing a flow channel, typically microscale in dimension, flowing at least two reagents into the flow channel and varying the flow rate of the mixture through the flow channel. By increasing and/or decreasing the flow rate of the reagent mixture from the point of mixing to the point of detection, one alters the amount of reaction time, allowing monitoring reaction kinetics over time.
Claims18
A method of monitoring a time dependent reaction, comprising:
- continuously flowing at least a first reagent through a first flow channel;
- initiating a reaction with the first reagent at a first point in the first flow channel;
- detecting a result of the reaction at a second point in the first flow channel;
- varying an amount of time between the initiation of the reaction and the detection with respect to the first reagent flowing through the first flow channel by varying a rate at which the first reagent flows through the first flow channel from the first point to the second point;
- and
- performing at least one detection at each of multiple flow rates, thereby monitoring a time dependent reaction.
3 more elements
Dependencies are inferred from the claim text.