Purification of red blood cells by separation and diafiltration
AssigneeBiopure Corporation
InventorsRobert A. Houtchens, Maria S. Gawryl, William R. Light, Javed Baqai
Life of this patent
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Abstract
Red blood cells are purified by separating whole blood, such as by centrifugation, to form a red blood cell fraction and a liquid fraction. The whole blood can be defibrinated or treated to prevent coagulation prior to separation. Preferably, the whole blood is bovine blood. The red blood cell fraction is then diafiltered to purify the red blood cells. The purified red blood cells can then be lysed to form a lysate of purified red blood cells. The purified red blood cells and the lysate of purified red blood cells are suitable for use in producing hemoglobin blood substitute.
Claims20
A method of purifying red blood cells, comprising the steps;
- a) separating defibrinated whole blood, whereby a red blood cell fraction and a liquid fraction are formed;
- and
- b) diafiltering the red blood cell fraction to thereby form purified red blood cells.
A method of forming a lysate of purified red blood cells for use in a hemoglobin blood substitute, comprising the steps;
- a) separating defibrinated whole blood, whereby a red blood cell fraction and a liquid fraction are formed;
- b) diafiltering the red blood cell fraction to thereby form purified red blood cells;
- and
- c) lysing the purified red blood cells, thereby forming the lysate of purified red blood cells.
A method of forming a lysate of purified red blood cells for use in a hemoglobin blood substitute, comprising the steps;
- a) separating defibrinated whole bovine blood by centrifugation, whereby a red blood cell fraction and a liquid fraction are formed;
- b) diafiltering the red blood cell fraction to thereby form purified red blood cells;
- and
- c) mechanically lysing the purified red blood cells, thereby forming the lysate of purified red blood cells.
Dependencies are inferred from the claim text.