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US 7,001,587 B2Granted
Measurement of protein synthesis rates in humans and experimental systems by use of isotopically labeled water
AssigneeThe Regents of the University of California
InventorMarc K. Hellerstein
Life of this patent
Estimating the term…
Abstract
Provided herein are methods for measuring protein biosynthesis by using 2H2O or radioactive 3H2O and applicable uses thereof.
Claims14
A method of determining the biosynthetic rate of one or more proteins or peptides in an individual comprising the steps of:
- (a) administering labeled water to an individual over a period of time sufficient for the label of said labeled water to be incorporated into said one or more proteins or peptides to form labeled and unlabeled proteins or peptides wherein said label is 2H, or 18O;
- (b) obtaining one or more bodily tissues or fluids from said individual, wherein said bodily tissues or fluids comprise said one or more labeled and unlabeled proteins or peptides;
- and
- (c) detecting the incorporation of said label in said one or more labeled proteins or peptides to determine the biosynthetic rate of said one or more proteins or peptides.
1 more element
A method of determining the biosynthetic rate of one or more proteins or peptides in an individual comprising the steps of:
- (a) administering labeled water to an individual over a period of time sufficient for the label of said labeled water to be incorporated into said one or more proteins or peptides and thereby form labeled and unlabeled proteins or peptides wherein said label is 2H or 18O;
- (b) obtaining one or more bodily tissues or fluids from said individual, wherein said bodily tissues or fluids comprise said one or more labeled and unlabeled proteins or peptides;
- (c) hydrolyzing said one or more labeled and unlabeled proteins to produce one or more labeled and unlabeled amino acids or oligopeptides;
- (d) detecting the incorporation of said label in said one or more labeled amino acids or oligopeptides to determine the biosynthetic rate of said one or more proteins or peptides.
1 more element
Dependencies are inferred from the claim text.