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US 7,001,550 B2Granted
Charged filtration membranes and uses therefor
AssigneeGenentech, Inc.
InventorRobert D. van Reis
Life of this patent
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Abstract
The invention relates to charged filtration membranes and their use for separation of a protein from solvent, low molecular weight solutes or a mixture of proteins. Modification of the membranes to generate charge includes modification of membrane pores to alter charge within a pore and alter the size of a pore. Consequently, the protein is separated from other solutes in a mixture based on size as well as net protein charge and membrane charge.
Claims4
A method of preparing a charged filtration membrane, the method comprising:
- contacting a precursor cellulose membrane with a reactive charged compound;
- and
- reacting a surface reactive hydroxyl group on the precursor membrane with a reactive group of the charged compound such that the charged compound is covalently attached to the membrane,
- wherein, membrane pore size distribution is reduced relative to the precursor membrane;
- wherein, for the charged membrane and a protein solute having like charge, solute sieving is improved by at least 1.5 fold relative to the precursor membrane, and
- wherein, a sieving versus permeability curve is shifted to lower sieving values, but the permeability values remain substantially the same, relative to a curve obtained with the precursor membrane.
3 more elements
Dependencies are inferred from the claim text.