Systems for column-based separations, methods of forming packed columns, and methods of purifying sample components

A method of purifying a component of a sample, comprising:

• providing a column chamber, the column chamber having an inlet end and an outlet end, the outlet end terminating proximate both a first flow path and a second flow path, the flow paths extending through a valve having at least two positions that controls flow in the flow paths, the first flow path being obstructed with a porous retaining material permeable to a first fluid and impermeable to a matrix material, flow in the second flow path blocked when the valve is in a first of the at least 2 positions, and flow in the first flow path blocked when the valve is in a second of the at least two positions;
• placing the valve in the first position;
• flowing the first fluid and the matrix material into the column chamber and along the first flow path to form a packed column of the matrix material within the column chamber, the matrix material being configured to selectively retain a component of the sample;
• flowing the sample through the packed column and along the first flow path to separate the component from the rest of the sample;
• placing the valve in the second position;
• and
• flowing a second fluid through the column chamber and along the second flow path to remove the matrix material from the column chamber.