Method for manufacturing glycoproteins having human-type glycosylation

A transformed plant cell having a sugar chain adding mechanism comprising a mammalian β1,4-galactosyltransferase enzyme which transfers a galactose residue to a non-reducing terminal acetylglucosamine residue, wherein the sugar chain adding mechanism adds a sugar chain containing a core sugar chain and an outer sugar chain, wherein the core sugar chain comprise a plurality of mannose and acetylglucosamine residues, and wherein the outer sugar chain contains a terminal sugar chain portion with a non-reducing terminal galactose.

A method of manufacturing a glycoprotein, which method includes introducing into a plant cell a gene encoding a β1,4-galactosyltransferase of human origin and further introducing a gene encoding an exogenous glycoprotein selected from the group consisting of enzymes, hormones, cytokines, antibodies, vaccines, receptors and serum proteins, the glycoprotein produced including a core sugar chain including a plurality of mannose and acetylglucosamine residues, and an outer sugar chain containing a terminal sugar chain portion with a non-reducing terminal galactose.