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US 6,998,258 B1Granted
L-pantolactone-hydrolase and a method for producing D-pantolactone
AssigneeBASF SE
InventorsMaria Kesseler, Bernhard Hauer, Thomas Friedrich, Ralf Mattes
Life of this patent
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Abstract
The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.
Claims20
An isolated nucleic acid sequence which codes for a polypeptide having L-pantolactone hydrolase activity, and which is useful for the preparation of D-pantolactone, selected from the group of:
- a) a nucleic acid sequence having the sequence depicted in SEQ ID NO:1,
- b) nucleic acid sequences which, as a result of the degeneracy of the genetic code, are derived from the nucleic acid sequence depicted in SEQ ID NO:1,
- c) derivatives of the nucleic acid sequence depicted in SEQ ID NO:1 which code for polypeptides having at least 80% homology at the amino acid level with the amino acid sequences depicted in SEQ ID NO:2, with negligible reduction in the enzymatic action of the polypeptides.
L-pantolactone hydrolase having the following properties:
- a) conversion of L-pantolactone into the corresponding acid,
- b) pH stability:
- L-pantolactone hydrolase is stable in a pH range from 4 to 10
- c) pH optimum:
- 7.2 to 7.6
- d) temperature optimum:
- about 70° C. to 75° C.
- e) no inhibition of the activity by EDTA.
5 more elements
Dependencies are inferred from the claim text.