Recombinant poxviruses having foreign DNA expressed under the control of poxvirus regulatory sequences
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsBernard Moss, Michael Mackett, Geoffrey L. Smith
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Abstract
Recombinant poxviruses, such as vaccinia, are provided that comprises a segment comprised of (A) a first DNA sequence encoding a polypeptide that is foreign to poxvirus and (B) a poxvirus transcriptional regulatory sequence, wherein (i) said transcriptional regulatory sequence is adjacent to and exerts transcriptional control over said first DNA sequence and (ii) said segment is positioned within a nonessential genomic region of said recombinant poxvirus. Vaccines, carriers, cells, and media comprising recombinant poxviruses, and methods of immunization with recombinant poxviruses also are provided.
Claims14
A plasmid that comprises (A) a segment comprised of (i) a first DNA sequence encoding a polypeptide that is foreign to poxvirus and (ii) a poxvirus promoter, wherein said promoter is adjacent to and exerts transcriptional control over said first DNA sequence;
- and, flanking said segment, (B) DNA from a nonessential region of a poxvirus genome.
A recombinant poxvirus that comprises a segment comprised of (A) a first DNA sequence encoding a polypeptide that is foreign to poxvirus and (B) a poxvirus promoter, wherein (i) said promoter is adjacent to and exerts transcriptional control over said first DNA sequence and (ii) said segment is positioned within a nonessential genomic region of said recombinant poxvirus.
A method for preparing a vector, comprised of the steps of (A) providing a plasmid and (B) incorporating into said plasmid a segment comprising a chimeric gene that contains (i) a first DNA sequence encoding a polypeptide that is foreign to poxvirus;
- (ii) a poxvirus promoter, wherein said promoter is adjacent to and exerts transcriptional control over said first DNA sequence;
- and (iii) DNA from a nonessential region of a poxvirus genome, said DNA flanking said chimeric gene such that said plasmid can undergo homologous recombination with a poxvirus genome in a poxvirus-infected cell.
A method for producing a polypeptide, comprising the steps of:
- (A) providing a recombinant poxvirus that contains a segment comprised of (i) a first DNA sequence encoding a polypeptide that is foreign to poxvirus and (ii) a poxvirus promoter, wherein (a) said promoter is adjacent to and exerts transcriptional control over said first DNA sequence and (b) said segment is positioned within a nonessential genomic region of said recombinant poxvirus;
- (B) infecting host cells with said recombinant poxvirus, such that said host cells express said polypeptide;
- and then
- (C) separating said polypeptide from said host cells.
Dependencies are inferred from the claim text.