Packaging cell
AssigneesChugai Seiyaku Kabushiki Kaisha, Toshio Kitamura
InventorsToshio Kitamura, Sumiyo Morita
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Abstract
A virus-producing cell sustaining the ability to produce viruses at high titer is successfully constructed by expressing the virus structural gene under the regulation of EF1α promoter. In this virus-producing cell, the virus structural gene is ligated to a selection marker gene via IRES and domains other than the protein coding domain are eliminated from the DNA encoding virus structural proteins. Thus, reduction of the titer due to cell passages can be prevented and emergence of wild type viruses caused by unfavorable recombination of the virus genome can be inhibited.
Claims17
A 293T cell useful for the production of retroviruses, wherein the cell contains an expression construct comprising DNA encoding gag, pol, and env retroviral structural proteins operably linked downstream of an EF1α promoter.
A 293T cell useful for the production of retroviruses by expressing retroviral structural proteins gag, pol and env, wherein the cell comprises a first expression construct expressing gag and pol from an EF1α promoter, and a second expression construct expressing env from an EF1α promoter.
A cell specified by Accession No. FERM BP-6737 or FERM BP-6977 as deposited at the National Institute of Bioscience and Human-Technology in Japan.
Dependencies are inferred from the claim text.