Reovirus clearance of ras-mediated neoplastic cells from mixed cellular compositions
AssigneeOncolytics Biotech Inc.
InventorsDonald Morris, Bradley G. Thompson, Matthew C. Coffey
Life of this patent
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Abstract
Reovirus can be used to selectively remove ras-mediated neoplastic cells from a cellular composition. It is of particular interest to purge autographs which may contain neoplastic cells with reovirus before transplanting the autographs back into the recipient, thereby reducing the risk of introducing or reintroducing neoplastic cells into the recipient.
Claims31
A method of preparing a cellular composition for transplantation into a recipient, comprising the steps of:
- (a) selecting a cellular composition for transplantation;
- and
- (b) contacting the composition with a reovirus ex viva to result in oncolysis of ras-mediated neoplastic cells.
A method of preparing a cellular composition for transplantation into a recipient, comprising the steps of:
- (a) selecting a cellular composition for transplantation;
- (b) contacting the composition with a reovirus ex viva to result in oncolysis of ras-mediated neoplastic cells;
- and
- (c) administering to the transplant recipient at least one substance selected from the group consisting of anti-reovirus antibodies and immune system stimulating agents.
A method of preparing a cellular composition that comprises hematopoletic stem cells harvested from blood for transplantation into a recipient, comprising contacting the cellular composition with a reovirus ex vivo to result in oncolysis of ras-mediated neoplastic cells without altering the ability of the hematopoietic cells to differentiate into each and every hematopoletic lineage.
Dependencies are inferred from the claim text.