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US 6,991,652 B2Granted
Tissue engineering composite
OwnerKaren J. L. Burginventor
Life of this patent
Estimating the term…
Abstract
The invention provides a biocompatible composite for use in a living subject for purposes of repairing damaged tissues and reconstructing a new tissue. The composite includes a biodegradable or absorbable three-dimensional support construct, a liquid or viscous fluid forming a gel matrix or viscous fluid when delivered to an area of interest in a living subject. The biodegradable construct provides an ideal surface for cell or cell extract attachment, while the gel matrix or viscous fluid acts as both a carrier material and a separator for maintaining the space between the constructs as well as the structural integrity of the developing issue.
Claims49
A composite for use into a living subject comprising:
- a biocompatible porous three-dimensional construct comprising a material selected from the group consisting of alginate, collagen, polylactide, polyethylene glycol, polycaprolactone, polycolide, polydioxanone, and derivatives and copolymers thereof and having a size no greater than about 1.5 mm in diameter distributed within a carrier,
- said carrier comprising a gel matrix or viscous fluid when injected into the body of a living subject said gel matrix or viscous fluid being selected from the group consisting of biodegradable materials and non-biodegradable materials, each having a viscosity of from 10 to 1000 cps, and
- cells disposed in said biocompatible three-dimensional construct,
- wherein the three-dimensional construct allows for the growth of the cells disposed therein and for the ingrowth and attachment of surrounding tissue cells of the living subject.
1 more element
A composite for use in a living subject comprising:
- a biocompatible three-dimensional construct comprising a material selected from the group consisting of alginate, collagen, polylactide, polyethylene glycol, polycaprolactone, polydioxanone, and derivatives, and copolymers thereof and having a diameter of about 1.5 mm or less distributed within a carrier,
- said carrier comprising a gel matrix or viscous fluid when injected into the body of a living subject, said gel matrix or viscous fluid being selected from the group consisting of biodegradable and non-biodegradable materials, each having a viscosity of from 10 to 1000 cps;
- and
- cells disposed in said carrier,
- such that when disposed within the living subject, the composite allows for growth of the cells disposed in said carrier and ingrowth of surround tissue cells.
2 more elements
A composite for use in a living subject comprising:
- a biocompatible porous three-dimensional construct comprising a material selected from the group consisting of alginate, collagen, polylactide, polyethylene glycol, polycaprolactone, polycolide, polydioxanone, and derivatives and copolymers thereof and having a size no greater than about 1.5 mm in diameter distributed within a carrier,
- said carrier comprising a liquid or viscous fluid capable of forming a gel matrix or viscous fluid when injected into the body of a living subject said gel matrix or viscous fluid being selected from the group consisting of biodegradable materials and non-biodegradable materials, each having a viscosity of from 10 to 1000 cps;
- and
- cells disposed in said biocompatible three-dimensional support construct and said carrier,
- wherein when disposed in the living subject, the composite allows for growth of the cells disposed therein and ingrowth of surround tissue cells.
2 more elements
A method of providing cells or tissue in a living subject comprising injecting into an area of interest in said living subject in need of treatment thereof an effective amount of a composite having a diameter of about 1.5 mm or less comprising:
- (a) a biocompatible three-dimensional support construct comprising a material selected from alginate, collagen, polylactide, polyethylene glycol, polycaprolactone, polydioxanone, and derivatives and copolymers thereof and having a porous structure which supports cell adhesion, growth and migration, thereby providing for ingrowth of surrounding tissue cells of the living subject;
- (b) a carrier comprising a liquid or viscous fluid forming a gel matrix or viscous fluid when delivered into the body of a living subject;
- and
- (c) cells disposed in said composite.
1 more element
A method for repairing a defect in the breast of a female patient, comprising:
- injecting into the breast area of a female patient in need of treatment thereof an effective amount of a composite comprising a biocompatible, biodegradable three-dimensional support construct having a diameter of about 1.5 mm or less distributed within a carrier and made of collagen and containing cells obtained from said patient and selected from the group consisting of fibroblasts, smooth muscle cells, endothelial cells, mesenchymal cells, and an alginate carrier, said carrier comprising a liquid or viscous fluid having a viscosity of from 10 to 1000 cps, such that upon injection the composite allows for the growth of the cells contained therein and for the ingrowth of surrounding tissue cells.
A method for repairing a defect in a breast of a female patient, comprising:
- injecting in the breast area of a female patient in need of treatment thereof an effective amount of a composite comprising a biocompatible, biodegradable three-dimensional support construct having a diameter of about 1.5 mm or less distributed within a carrier and made of collagen and containing cells obtained from a cell bank and selected from the group consisting of fibroblasts, smooth muscle cells, endothelial cells, mesenchymal cells, and an alginate carrier, said carrier comprising a liquid or viscous fluid having a viscosity of from 10 to 1000 cps, such that upon injection the composite allows for the growth of the cells contained therein and for the ingrowth of surrounding tissue cells.
A method for repairing a defect in a breast of a female patient, comprising:
- injecting in the breast area of a female patient in need of treatment thereof an effective amount of a composite comprising a biocompatible, biodegradable three-dimensional support construct having a diameter of about 1.5 mm or less distributed within a carrier and made of polylactide based material and containing cells obtained from said patient and selected from the group consisting of fibroblasts, smooth muscle cells, endothelial cells, mesenchymal cells, and an alginate carrier, said carrier comprising a liquid or viscous fluid having a viscosity of from 10 to 1000 cps, such that upon injection the composite allows for the growth of the cells contained therein and for the ingrowth of surrounding tissue cells.
A method for repairing a facial defect in a patient, comprising:
- injecting into the face of a patient in need of treatment thereof an effective amount of a composite comprising a biocompatible, biodegradable three-dimensional support construct having a diameter of about 1.5 mm or less distributed within a carrier and made of collagen and containing cells obtained from said patient and selected from the group consisting of fibroblasts, smooth muscle cells, endothelial cells, mesenchymal cells, and an alginate carrier, such that upon injection the composite allows for the growth of the cells contained therein and for the ingrowth of surrounding tissue cells.
A method for repairing a facial defect in a patient, comprising:
- injecting into the face of a patient in need of treatment thereof an effective amount of a composite comprising a biocompatible, biodegradable porous three-dimensional support construct having a diameter of about 1.5 mm or less and made from collagen and an alginate carrier, such that upon injection the porous support construct allows for ingrowth of surrounding tissue cells.
A method for repairing a defect in the nipple of a female patient, comprising:
- injecting into the nipple area of the breast of a patient in need of treatment thereof an effective amount of a composite comprising a biocompatible, biodegradable porous three-dimensional construct having a diameter of about 1.5 mm or less and of collagen, and an alginate carrier, such that upon injection the porous support construct allows for ingrowth of surrounding tissue cells.
A method for repairing a spinal disc in a patient, comprising:
- injecting into the spinal disc a composite comprising a biocompatible, biodegradable porous three-dimensional construct having a diameter of about 1.5 mm or less and of collagen, said construct containing intervertebral disc cells obtained from said patient, and an alginate carrier, such that upon injection the composite allows for the growth of the cells contained therein and for the ingrowth of surrounding tissue cells.
A method for repairing a defect in a bone of a patient, comprising:
- injecting into the bone of a patient in need of treatment thereof an effective amount of a composite comprising a biocompatible, biodegradable three-dimensional construct having a diameter of about 1.5 mm or less and made from polylactide osteoblast cells obtained from said patient, and an alginate, such that upon injection the composite allows for the growth of the cells contained therein and for the ingrowth of surrounding tissue cells.
Dependencies are inferred from the claim text.