Multilayered microfluidic DNA analysis system and method

A multilayered microfluidic DNA amplification device comprising:

• a monolithic structure formed from a plurality of green-sheet layers sintered together, said green-sheet layers including particles selected from the group consisting of ceramic particles, glass particles, and glass-ceramic particles;
• said monolithic structure having a fluid passageway defined therein, said fluid passageway including an inlet port for receiving the fluid, a cell lysis chamber for receiving the fluid from the inlet port, a DNA separation chamber, a buffer injection port and a first waste outlet port coupled to the DNA separation chamber, a DNA amplification chamber, a regent injection port and a second waste outlet port coupled to the DNA amplififcation chamber, and a DNA detection chamber;
• one or more electric field generation elements positioned in the cell lysis chamber;
• a first fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid from the cell lysis chamber to the DNA separation chamber;
• a magnetic field element contiguous to the DNA separation chamber;
• a second fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid from the DNA seperation chamber to the DNA amplififcation chamber;
• a heating and cooling element contiguous to the DNA amplification chamber for subjecting the DNA to a plurality of cycles of a temperature regimen;
• a third fluid control system contiguous to the fluid passageway for providing electroosmotic pumping to transport the fluid from the DNA amplification chamber to the DNA detection chamber;
• and
• a transparent material positioned adjacent the DNA detection chamber allowing optical access thereto.